Coding

Part:BBa_K3132010

Designed by: Qiliang Yang   Group: iGEM19_SMMU-China   (2019-10-06)


STAT3 transcription factor

The intracellular domain of JAK/STAT-GEMS IL-6RB activates JAKs phosphorylate STAT3, and then STAT3 functions as transcription factor. After phosphorylated, STAT3 polymerizes into a dimer form as activated transcription factor that enters the cell nucleus and binds to a specific binding site of the target gene promoter sequence to promote transcription. It consists of six structural domains: a sequence-specific DNA binding domain, a transcription activation domain, N terminal conservative sequence associated with dimerization,curly spiral domain supporting interaction with other transcription factors, LD domain participating regulation of transcription and SH2 domain with high conservative.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2285
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1637
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1337


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Categories
Parameters
None